Understanding lncRNA-protein assemblies with imaging and single-molecule approaches.
Curr Opin Genet Dev
; 72: 128-137, 2022 02.
Article
in En
| MEDLINE
| ID: mdl-34933201
ABSTRACT
Long non-coding RNAs (lncRNAs) associate with RNA-binding proteins (RBPs) to form lncRNA-protein complexes that act in a wide range of biological processes. Understanding the molecular mechanism of how a lncRNA-protein complex is assembled and regulated is key for their cellular functions. In this mini-review, we outline molecular methods used to identify lncRNA-protein interactions from large-scale to individual levels using bulk cells as well as those recently developed imaging and single-molecule approaches that are capable of visualizing RNA-protein assemblies in single cells and in real-time. Focusing on the latter group of approaches, we discuss their applications and limitations, which nevertheless have enabled quantification and comprehensive dissection of RNA-protein interactions possible.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
RNA-Binding Proteins
/
RNA, Long Noncoding
Language:
En
Journal:
Curr Opin Genet Dev
Journal subject:
GENETICA
Year:
2022
Document type:
Article